Products related to Microscopy:
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Insect Microscopy
Insects, and their close relatives, the arachnids, centipedes, millipedes and woodlice, make ideal material for study by the recreational microscopist. Moreover for the entomologist, the addition of the use of the microscope to their tool kit adds a whole new dimension to their study, revealing in finest detail the appearance and structure of these tiny creatures. This book reveals the basics of insect microscopy, explaining what equipment is needed and how to get the best out of it. Topics covered include insects and their relatives; trapping insects for study; dissection, slide mounting publishing your work.
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Light Sheet Fluorescence Microscopy
Light Sheet Fluorescence Microscopy An indispensable guide to a novel, revolutionary fluorescence microscopy technique! Light sheet fluorescence microscopy has revolutionized microscopy, since it allows scientists to perform experiments in an entirely different manner and to record data that had not been accessible before.With contributions from noted experts in the fields of physics, biology, and computer science, Light Sheet Fluorescence Microscopy is a unique guide that offers a practical approach to the subject, including information on the basics of light sheet fluorescence microscopy, instrumentation, applications, sample preparation, and data analysis.Comprehensive in scope, the book is filled with the cutting-edge methods as well as valuable insider tips.Grounded in real-world applications, the book includes chapters from major manufacturers that explores their recent systems and developments.In addition, the book hightlights a discussion of a “do-it-yourself” light sheet microscope, making the technique affordable for every laboratory.This important textbook: Serves as an easy-to-understand introduction to light sheet-based fluorescenceIncludes numerous tips and tricks for advanced practitionersProvides in-depth information on hardware and software solutions for a straightforward implementation of light sheet fluorescence microscopy in the lab Includes chapters from the major manufacturers including Zeiss, Leica, Lavision Biotech, Phase View, and Asi Aimed at cell biologists, biophysicists, developmental biologists, and neuro-biologists, Light Sheet Fluorescence Microscopy offers a comprehensive overview of the most recent applications of this microscopy technique.
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Microscopy of the Heart
This book provides in depths information on different microscopy approaches and supplies the reader with methods how to untangle highly complex processes involved in physiological and pathophysiological cardiac signaling.Microscopy approaches have established themselves as the quasi gold standard that enables us to appreciate the underlying mechanisms of physiological and pathophysiological cardiac signaling.This book presents the most important microscopy techniques from the level of individual molecule e.g.Förster-Resonance Energy Transfer (FRET), up to cellular and tissue imaging, e.g. electron microscopy (TEM) or light sheet microscopy.The book is intended for graduate students and postdocs in cardiovascular research, imaging and cell biology, pre-clinical and clinical researchers in cardiovascular sciences as well as decision makers of the pharmaceutical industry.
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Confocal Microscopy for Biologists
There has been a great upsurge in interest in light microscopy in recent years due to the advent of a number of significant advances in microscopy, one of the most important of which is confocal microscopy.Confocal microscopy has now become an important research tool, with a large number of new fluorescent dyes becoming available in the past few years, for probing your pet structure or molecule within fixed or living cell or tissue sampies.Many of the people interested in using confocal microscopy to further their research do not have a background in microscopy or even cell biology and so not only do they find considerable difficulty in obtaining satisfactory results with a confocal microscope, but they may be mislead by how data is being presented.This book is intended to teach you the basic concepts ofmicroscopy, fluorescence, digital imaging and the principles of confocal microscopy so that you may take full advantage ofthe excellent confocal microscopes now available.This book is also an excellent reference source for information related to confocal microscopy for both beginners and the more advanced users.For example, do you need to know the optimal pinhole size for a 63x 1. 4 NA lens? Do you need to know the fluorescence emission spectrum of Alexa 568?Access to the wealth of practical information in this book is made easier by using both the detailed index and the extensive glossary.
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How is electron microscopy stained?
Electron microscopy uses heavy metal stains to enhance contrast and visibility of the specimen. These stains include uranyl acetate and lead citrate, which are applied to the specimen after it has been fixed and dehydrated. The heavy metal stains interact with the electrons in the microscope, creating contrast between different structures within the specimen. This allows for detailed imaging of the ultrastructure of cells and tissues at a very high resolution.
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Is there a timer for mindfulness training, meditation, yoga, and spirituality?
There is no set timer for mindfulness training, meditation, yoga, and spirituality as it varies from person to person. Some people may find it helpful to start with short sessions, such as 5-10 minutes, and gradually increase the duration as they become more comfortable. Others may prefer longer sessions right from the start. It's important to listen to your body and mind and find a timing that works best for you. The key is to be consistent and make it a regular practice in your daily routine.
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What is the command for microscopy?
The command for microscopy is typically "microscope on" or "turn on the microscope." This command is used to activate the microscope and prepare it for use. Once the microscope is turned on, the user can then adjust the settings and focus to begin examining the specimen.
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How can I obtain tardigrades for microscopy?
Tardigrades can be obtained for microscopy by collecting samples from mosses, lichens, and other damp environments where they are known to thrive. Once collected, the samples can be placed in a petri dish with a small amount of water and observed under a microscope. Tardigrades are known for their ability to survive extreme conditions, so it is important to ensure that the environment in the petri dish remains suitable for their survival. Additionally, there are commercial suppliers that sell tardigrades for microscopy purposes.
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Introduction to Analytical Electron Microscopy
The birth of analytical electron microscopy (AEM) is somewhat obscure.Was it the recognition of the power and the development of STEM that signaled its birth?Was AEM born with the attachment of a crystal spectrometer to an otherwise conventional TEM? Or was it born earlier with the first analysis of electron loss spectra?It's not likely that any of these developments alone would have been sufficient and there have been many others (microdiffraction, EDS, microbeam fabrication, etc.) that could equally lay claim to being critical to the establishment of true AEM.It is probably more accurate to simply ascribe the present rapid development to the obvious: a combination of ideas whose time has come.Perhaps it is difficult to trace the birth of AEM simply because it remains a point of contention to even define its true scope.For example, the topics in this book, even though very broad, are still far from a complete description of what many call AEM.When electron beams interact with a solid it is well-known that a bewildering number of possible interactions follow.Analytical electron microscopy attempts to take full qualitative and quantitative advantage of as many of these interactions as possible while still preserving the capability of high resolution imaging.Although we restrict ourselves here to electron transparent films, much of what is described applies to thick specimens as well.Not surprisingly, signals from all possible interactions cannot yet (and probably never will) be attained simultaneously under optimum conditions.
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Microscopy : A Very Short Introduction
Microscopy is a dynamic area of science, incorporating both basic classroom microscopes and sophisticated research style instruments that can be driven by light, electrons, or X-rays.The rate of advance in the area over the last 50 years has led to a number of technological advances.In this Very Short Introduction Terence Allen, an established expert on microscope techniques, describes the scientific principles behind the main forms of microscopy, and the exciting new developments in the field.Focusing on the main underlying principles, and introducing the power of what is achievable today using microscopes, Allen demonstrates how microscopy impinges on almost every aspect of our daily lives; from medical diagnosis to quality control in manufacture.Beginning with a brief history of the early stages of microscopy development, Allen then concludes with a comprehensive account of the diverse spectrum of microscopy available today.ABOUT THE SERIES: The Very Short Introductions series from Oxford University Press contains hundreds of titles in almost every subject area.These pocket-sized books are the perfect way to get ahead in a new subject quickly.Our expert authors combine facts, analysis, perspective, new ideas, and enthusiasm to make interesting and challenging topics highly readable.
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Introduction to Confocal Fluorescence Microscopy
This book provides a comprehensive account of the theory of image formation in a confocal fluorescence microscope as well as a practical guideline to the operation of the instrument, its limitations, and the interpretation of confocal microscopy data.The appendices provide a quick reference to optical theory, microscopy-related formulas and definitions, and Fourier theory.
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Super-Resolution Microscopy : A Practical Guide
This unique book on super-resolution microscopy techniques presents comparative, in-depth analyses of the strengths and weaknesses of the individual approaches.It was written for non-experts who need to understand the principles of super-resolution or who wish to use recently commercialized instruments as well as for professionals who plan to realize novel microscopic devices.Explaining the practical requirements in terms of hardware, software and sample preparation, the book offers a wealth of hands-on tips and practical tricks to get a setup running, provides invaluable help and support for successful data acquisition and specific advice in the context of data analysis and visualization.Furthermore, it addresses a wide array of transdisciplinary fields of applications. The author begins by outlining the joint efforts that have led to achieving super-resolution microscopy combining advances in single-molecule photo-physics, fluorophore design and fluorescent labeling, instrument design and software development.The following chapters depict and compare current main standard techniques such as structured illumination microscopy, single-molecule localization, stimulated emission depletion microscopy and multi-scale imaging including light-sheet and expansion microscopy.For each individual approach the experimental setups are introduced, the imaging protocols are provided and the various applications illustrated.The book concludes with a discussion of future challenges addressing issues of routine applications and further commercialization of the available methods. Guiding users in how to make choices for the design of their own experiments from scratch to promising application, this one-stop resource is intended for researchers in the applied sciences, from chemistry to biology and medicine to physics and engineering.
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Can you explain phase contrast microscopy simply?
Phase contrast microscopy is a technique used to enhance the contrast of transparent, unstained samples, such as living cells or tissues, under a microscope. It works by taking advantage of the phase differences between light waves passing through different parts of the sample, converting these differences into variations in brightness and darkness. This allows for the visualization of internal structures and details that would otherwise be difficult to see with traditional brightfield microscopy. In simple terms, phase contrast microscopy makes transparent samples appear more visible and detailed under the microscope.
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How can I get tardigrades for microscopy?
Tardigrades can be found in various environments such as moss, lichen, leaf litter, and soil. To collect tardigrades for microscopy, you can start by collecting samples from these habitats using a small brush or pipette. The samples can then be placed in a petri dish with a small amount of water and observed under a microscope. Tardigrades are known for their ability to survive extreme conditions, so make sure to keep the samples moist and at a suitable temperature during observation.
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What is the purpose and necessity of microscopy?
The purpose of microscopy is to observe and study objects that are too small to be seen with the naked eye. Microscopy allows scientists and researchers to examine the structure, composition, and behavior of microscopic organisms, cells, tissues, and materials. It is necessary for various scientific fields such as biology, medicine, materials science, and nanotechnology, as it provides valuable insights into the fundamental building blocks of life and matter. Microscopy also plays a crucial role in diagnosing diseases, developing new technologies, and advancing our understanding of the natural world.
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What are the advantages of cryo-electron microscopy?
Cryo-electron microscopy (cryo-EM) offers several advantages over other imaging techniques. Firstly, it allows for the visualization of biological samples in their native state without the need for staining or fixing, providing more accurate structural information. Secondly, cryo-EM can achieve higher resolution images compared to traditional electron microscopy, making it a powerful tool for studying complex biological structures. Lastly, cryo-EM is a versatile technique that can be used to study a wide range of samples, from small molecules to large macromolecular complexes.
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